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S. sonnei and S. <t>flexneri</t> <t>2a</t> bacterial (CFUs, in %) invasion into HeLa cells after treatment with sera of the mice immunized with VirG epitope fusion proteins, based on antibody invasion inhibition assays. Shigella bacteria (2 × 10 7 CFUs) after incubation with mouse sera (30 µL, heat-inactivated, pooled from each group intramuscularly immunized with an individual epitope fusion protein) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ), then treated with gentamycin to remove extracellular bacteria, lysed, diluted, and plated on agar plates for overnight culture. Invaded bacterial CFUs were converted to percentage based on the invaded bacteria treated with the control sera as 100%. Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.
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S. sonnei and S. <t>flexneri</t> <t>2a</t> bacterial (CFUs, in %) invasion into HeLa cells after treatment with sera of the mice immunized with VirG epitope fusion proteins, based on antibody invasion inhibition assays. Shigella bacteria (2 × 10 7 CFUs) after incubation with mouse sera (30 µL, heat-inactivated, pooled from each group intramuscularly immunized with an individual epitope fusion protein) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ), then treated with gentamycin to remove extracellular bacteria, lysed, diluted, and plated on agar plates for overnight culture. Invaded bacterial CFUs were converted to percentage based on the invaded bacteria treated with the control sera as 100%. Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.
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S. sonnei and S. <t>flexneri</t> <t>2a</t> bacterial (CFUs, in %) invasion into HeLa cells after treatment with sera of the mice immunized with VirG epitope fusion proteins, based on antibody invasion inhibition assays. Shigella bacteria (2 × 10 7 CFUs) after incubation with mouse sera (30 µL, heat-inactivated, pooled from each group intramuscularly immunized with an individual epitope fusion protein) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ), then treated with gentamycin to remove extracellular bacteria, lysed, diluted, and plated on agar plates for overnight culture. Invaded bacterial CFUs were converted to percentage based on the invaded bacteria treated with the control sera as 100%. Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.
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S. sonnei and S. <t>flexneri</t> <t>2a</t> bacterial (CFUs, in %) invasion into HeLa cells after treatment with sera of the mice immunized with VirG epitope fusion proteins, based on antibody invasion inhibition assays. Shigella bacteria (2 × 10 7 CFUs) after incubation with mouse sera (30 µL, heat-inactivated, pooled from each group intramuscularly immunized with an individual epitope fusion protein) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ), then treated with gentamycin to remove extracellular bacteria, lysed, diluted, and plated on agar plates for overnight culture. Invaded bacterial CFUs were converted to percentage based on the invaded bacteria treated with the control sera as 100%. Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.
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S. sonnei and S. flexneri 2a bacterial (CFUs, in %) invasion into HeLa cells after treatment with sera of the mice immunized with VirG epitope fusion proteins, based on antibody invasion inhibition assays. Shigella bacteria (2 × 10 7 CFUs) after incubation with mouse sera (30 µL, heat-inactivated, pooled from each group intramuscularly immunized with an individual epitope fusion protein) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ), then treated with gentamycin to remove extracellular bacteria, lysed, diluted, and plated on agar plates for overnight culture. Invaded bacterial CFUs were converted to percentage based on the invaded bacteria treated with the control sera as 100%. Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.

Journal: Applied and Environmental Microbiology

Article Title: Characterization of Shigella virulence factor intracellular spread A (IcsA, or VirG) functional epitopes against S. flexneri 2a and S. sonnei invasion and adherence

doi: 10.1128/aem.01175-25

Figure Lengend Snippet: S. sonnei and S. flexneri 2a bacterial (CFUs, in %) invasion into HeLa cells after treatment with sera of the mice immunized with VirG epitope fusion proteins, based on antibody invasion inhibition assays. Shigella bacteria (2 × 10 7 CFUs) after incubation with mouse sera (30 µL, heat-inactivated, pooled from each group intramuscularly immunized with an individual epitope fusion protein) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ), then treated with gentamycin to remove extracellular bacteria, lysed, diluted, and plated on agar plates for overnight culture. Invaded bacterial CFUs were converted to percentage based on the invaded bacteria treated with the control sera as 100%. Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.

Article Snippet: Briefly, S. sonnei or S. flexneri 2a Congo red strain bacteria (~ 2 × 10 7 CFUs in 30 μL PBS) were mixed with 30 μL heat-inactivated (56°C for 30 min) mouse sera, which were pooled from each group, and incubated at room temperature for 25 min, and then transferred to >95% confluent monolayered HeLa cells (ATCC, CCL-2) on a 24-well culture plate.

Techniques: Inhibition, Bacteria, Incubation, Control

S. flexneri 2a bacterial (CFUs, in %) adherence and/or invasion to HeLa cells after treatment with sera of the mice immunized with fusion proteins with VirG epitope #2, #3, #7, #9, or #10. S. flexneri 2a bacteria (1.8 × 10 8 CFUs) after incubation with mouse sera (30 µL, pooled from each group immunized with an individual fusion protein, heat-inactivated) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ). After incubation, one group was treated with gentamicin to calculate the number of invaded bacteria, and the other was not to measure the number of adherent and invaded bacteria. Subtraction of the invaded bacteria from the adhered and invaded bacteria resulted in the adherent bacteria (CFUs). Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.

Journal: Applied and Environmental Microbiology

Article Title: Characterization of Shigella virulence factor intracellular spread A (IcsA, or VirG) functional epitopes against S. flexneri 2a and S. sonnei invasion and adherence

doi: 10.1128/aem.01175-25

Figure Lengend Snippet: S. flexneri 2a bacterial (CFUs, in %) adherence and/or invasion to HeLa cells after treatment with sera of the mice immunized with fusion proteins with VirG epitope #2, #3, #7, #9, or #10. S. flexneri 2a bacteria (1.8 × 10 8 CFUs) after incubation with mouse sera (30 µL, pooled from each group immunized with an individual fusion protein, heat-inactivated) were transferred to and incubated with HeLa cells (~2.5 × 10 5 ). After incubation, one group was treated with gentamicin to calculate the number of invaded bacteria, and the other was not to measure the number of adherent and invaded bacteria. Subtraction of the invaded bacteria from the adhered and invaded bacteria resulted in the adherent bacteria (CFUs). Boxes and bars indicate CFU means and standard deviations; *** for P < 0.001.

Article Snippet: Briefly, S. sonnei or S. flexneri 2a Congo red strain bacteria (~ 2 × 10 7 CFUs in 30 μL PBS) were mixed with 30 μL heat-inactivated (56°C for 30 min) mouse sera, which were pooled from each group, and incubated at room temperature for 25 min, and then transferred to >95% confluent monolayered HeLa cells (ATCC, CCL-2) on a 24-well culture plate.

Techniques: Bacteria, Incubation